HEXOKINASE TYPE II; RBX-100UL

Analysis Note

Controlinsulin-responsive tissues such as skeletal muscle

Application

Research Sub CategoryEnzymes & Biochemistry

Research CategoryMetabolism

Western Blot: 1:5,000-1:10,000.

Immunocytochemistry using indirect immunofluorescence.

ELISA

Optimal working dilutions must be determined by the end user.

Anti-Hexokinase Type II Antibody is an antibody against Hexokinase Type II for use in ELISA, IC & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant rat Type II hexokinase.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid. Partially Purified rabbit Serum. Antibodies reactive with the Type I isozyme were present in the original antiSerum; these were removed by affinity chromatography on a column of immobilized Type I isozyme, and the affinity-depleted Serum restored to original volume.

Immunodepleted Serum

Format: Purified

Specificity

Reacts with the Type II isozyme of rat hexokinase (102 kDa). No reactivity with the Type I or Type III isozymes of rat hexokinase by immunoblot or ELISA. Reactivity with human and hamster Type II isozyme has been confirmed by immunoblotting, and reactivity with Type II isozyme from other mammalian species is expected.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

102 kDa